|
KMID : 0545120020120030470
|
|
Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.470 ~ p.475
|
|
|
Isolation and Biological Properties of Novel Cell Cycle Inhibitor, HY558, Isolated from Penicillium minioluteum F558
|
|
Lee, Chul Hoon
Lim, Hae Young/Kim, Min Kyoung/Cho, Youl Hee/Oh, Deok Kun/Kim, Chang Jin/Lim, Yoong Ho
|
|
|
Abstract
|
|
|
|
In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of C_20H_44ON_2. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its IC_50 values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL60, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, HY558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with HY558. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|